Direct electron detector camera for cryo-EM

The Thermo Scientific Falcon 4i Direct Electron Detector is the ideal solution for your single particle analysis  and  cryo-tomography applications. With its unique combination of high image quality, high throughput, efficient lossless data compression enabled via electron-event representation (EER), and a streamlined solution for data management and quality monitoring, the Falcon 4i Detector offers a productivity and performance boost that meets the demands of today’s scientific and industrial communities.

The Falcon 4i Detector is fully integrated in Thermo Scientific EPU and Tomography Software, allowing for smooth daily instrument operation and data acquisition. The camera’s output is recorded through the Data Management Platform (DMP), which also facilitates the organization, viewing, and sharing of single particle cryo-EM data. You can easily view all the project data and metadata at the microscope or remotely through a secure connection, and can comment and share it with collaborators.

Note: The Falcon 4i Direct Electron Detector is fully compatible with 200 and 300 kV Thermo Scientific Krios, Glacios, and Talos Cryo-Transmission Electron Microscopes (Cryo-TEMs) operating with Windows 10. A Falcon 4i Upgrade Kit is available for the Falcon 4 Direct Electron Detector. The Falcon 4i Upgrade Kit upgrade is also applicable for the Thermo Scientific Selectris and Selectris X Imaging Filters.

Apoferritin reconstruction at 1.2 Å resolution created from 3,600 images of 297k particles acquired in 6 hours (EPU data acquisition on the Thermo Scientific Krios G4 Cryo-TEM). Recorded at a dose rate of 5.4 e/p/s.

Amyloid-β 42 filaments isolated from human brain resulting in a 2.5 Å resolution reconstruction. Data collected at dose rate 8 e/p/s, 40 e/A2 total dose and 570 movies/hr. Collaboration with MRC Laboratory of Molecular Biology. doi: https://doi.org/10.1101/2021.10.19.464936